Course Contents

COURSE CONTENTS - 1. SALIENT FEATURES OF CLONING VECTORS a. Types of cloning vectors b. Plasmids, cosmids, Yeast cloning vectors c. Animal viruses, Ti plasmids . 2. PLASMIDS a Structural and functional organization of plasmids b. Plasmid replication c. Stringent and relaxed plasmids d. Incompatibility of plasmid maintenance - 3. GENE MODIFICATION & APPLICATION OF RECOMBINANT DNA TECHNOLOGY a. Mutagenesis-Deletion mutagenesis, Oligonucleotide derived mutagenesis, Site directed mutagenesis b. Applications of rDNA technology in Diagnostics c. Molecular probes (Production, labelling and uses) - 4. ENZYMES IN GENETIC ENGINEERING a. DNA polymerase, polynucleotide kinase, T4 DNA ligase b. Nick translation system c. Terminal deoxynucleotidy1 transferase, Reverse transcriptase, Restriction endonucleases Type I & II. Genomic and cDNA libraries, screening methods for gene libraries; Southern and Northern blotting; Potentials of recombinant DNA technology; PCR; production of proteins,transgenic organisms and gene therapies Books Recommended 1. Basic Biotechnology. C. R. Attedge, and B. Kristiansen, 2001. Cambridge University Press, U.K. 2. A Manual of Basic Technique. Freshney, R. Ian. 2000. Alan R. Liss, Inc., New York. 3. Application of Microbes in Biotechnology. Lee, Y.K. & C.L. Poh and H. M. Tan.1999. Springer-verlag.