Course Contents

COURSE CONTENTS Chemical, Physical and Biological Properties of DNA. Structure and chemical composition of a gene, Types of gene. DNA polymerases, their types in eukaryotes and functions. RNA, its types and structures, Types of RNA polymerases in prokaryotes and in eukaryotes. Gene expression and regulation in both prokaryotes and eukaryotes. Mutation, mutagenes, Chemical, physical and Biological mutagenes, DNA repair. Transposons, its types, mechanism of transposition. Recombinant DNA Technology, Restriction enzymes, types of restriction enzymes and their mode of action. Cloninig vectors, Plasmids as cloning vectors, bacteriophage lambda as cloning vector, Cosmids as cloning vectors and shuttle vectors. Synthesis of cDNA, Cloning in Ecoli, Yeast and in Higher eukaryotes. Anti-sense RNA, Tripple helix DNA and chromosomal walking. Applications of recombinant DNA technology. PRACTICALS Demonstration of the related equipments and working of these equipments present in the department and University Central Laboratory.

Course Learning Outcomes

AIMS AND OBJECTIVES • An understanding of how molecular mechanisms are constructed and regulated knowledge about different molecular biological techniques