Course Contents

Extraction, Fractionation and Purification of Macromolecules Homogenization, Solubilization and Concentration including ultrasonication, lyphilization and ultradecantation, Purification based on differential solubility techniques, Ion-Exchange chromatography, Gel chromatography, Affinity chromatography, Paper & Thin layer chromatography and HPLC. Electrophoresis Paper and Gel electrophoresis.Two-dimensional electrophoresis.Capillary electrophoresis. Elecrofocusing Preparative and Analytical electrofocusing. Centrifugation Principle.Preparative centrifugation.Application of density gradient and differential centrifugation.Ultracentrifugation.Sedimentation equilibrium and sedimentation velocity methods.Application of analytical centrifugation. Tracer techniques Detection and measurement of radioactivity.Application of radioisotopes in biological system. Immunochemical Techniques

Course Synopsis

This course is very important for biochemistry students as it includes theoretical knowledge of important techniques commonly used in biochemistry lab and research.

Course Learning Outcomes

By the end of this course, students will have a deep knowledge of various basic as well as advance biochemical techniques like, sonication, chromatography, electrophoresis,ELISA, PCR and other immunological techniques.