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Course Contents

Introduction to recombinant DNA technology; restriction and modifying enzymes; cloning and expression vectors and their types; expression of recombinant proteins and their purification by affinity chromatography; polymerase chain reaction (PCR) - types; (inverse, touch-down, nested, hemi-nested, pit stop, multiplex, reverse transcriptase, RACE, real-time) and its applications; detection of mutations and/or SNPs; DNA fingerprinting; analysis of nucleic acids by gel electrophoresis – horizontal, vertical, pulse field, denaturing gradient gel electrophoresis; analysis of proteins by native and SDS-PAGE; 2-D gels; generation of antibodies and their uses; enzyme-linked immunosorbant assay; Southern, Western, Northern blotting.

Course Synopsis

To acquaint students with the experimental aspects of molecular biology

Course Learning Outcomes

students will be able to learn and perform the experimental aspects of molecular biology.

Introduction to recombinant DNA technology

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restriction and modifying enzymes

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cloning and expression vectors

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expression of recombinant proteins and their purification by affinity chromatography

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polymerase chain reaction (PCR)

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Book Title : Molecular Biology Techniques
Author : Sue Carson Heather Miller Melissa Srougi D. Scott Witherow
Edition : 4
Publisher : elsevier
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Title : Recombinant DNA technology
Type : Other

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Title : vectors
Type : Other

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Title : protein purification
Type : Other

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