Course Contents

Introduction to various analytical techniques; principles and applications of various types of chromatography including paper, thin layer, gel filtration, ion-exchange, affinity, high performance liquid chromatography (HPLC), gas chromatography, GC-MS and LC–MS; spectroscopy types including nuclear magnetic resonance (NMR), visible, ultraviolet, luminescence, flame, atomic absorption, fluorescence, emission and inductively coupled plasma emission spectroscopy (ICPMS); principles and applications of flow cytometry; introduction to X-ray diffraction; general analytical instrumentations and methods of fractionation and characterization of proteins and nucleic acids including dialysis, ultra-filtration, lyophilisation, ultracentrifuge and amino acid analyzer. PRACTICALS Separation of biomolecules by paper, column and thin layer chromatography; determination of molecular weight of proteins by gel filtration; identification of sugars, proteins, electrolytes etc. by UV/Visible spectrophotometer; determination of sodium and potassium content in blood serum by flame photometer and mineral analysis of plant tissues using atomic absorption spectrophotometer. RECOMMENDED TEXTBOOKS 1. Boyer RF, 2011. Biochemistry Laboratory: Modern Theory and Techniques. Second Edition; Prentice Hall 2. Wilson K, 2010. Principles and Techniques of Biochemistry and Molecular Biology. Seventh Edition; Cambridge University Press. 3. Christian GD, 2003. Analytical Chemistry. Sixth Edition, John Wiley and Sons, New York. 4. Chung et al., 2005. Analytical Methods validation and Instrument Performance verification. First Edition; John Wiley and Sons, New York. 5. Sharma BK, 2005. Instrumental Method of Chemical analysis. First Edition; Meerut Goel Publishing House, India. 6. Harris DC, 2010. Quantitative Chemical analysis. Eighth Edition; WH Freeman, New York.

Course Synopsis

To acquaint students with key analytical chemistry concepts involving identification and analysis at the molecular level by introducing a variety of analytical chemistry techniques and their applications at the molecular level; designing analytical chemistry methods to obtain analysis data with the high precision and accuracy from experiments; demonstrating biochemical laboratory techniques and explaining the theory and background behind these techniques.

Course Learning Outcomes

To acquaint students with key analytical chemistry concepts involving identification and analysis at the molecular level by introducing a variety of analytical chemistry techniques and their applications at the molecular level; designing analytical chemistry methods to obtain analysis data with the high precision and accuracy from experiments; demonstrating biochemical laboratory techniques and explaining the theory and background behind these techniques.