Course Contents
Organization and structure of genomes; genetic mapping (RFLP, microsatellite, SNP); high-resolution physical mapping (STS, EST); flow cytometry; somatic cell and radiation hybrids; artificial chromosomes in bacteria and yeast; hierarchical and whole genome shotgun sequencing; DNA sequencing strategies - manual and automated sequencing, pyrosequencing, Solexa, Helicos, Roche 454, real-time and nanopore sequencing; sequence assembly, obstacles and solutions; estimating gene number – over-prediction and under-prediction, homology searches, exon prediction programs, integrated gene-finding software packages; structural variation in the genome and its applications; microarray and RNA interference; proteomics; cellular communication/signaling pathways; protein-protein interactions and validation - yeast two hybrid system, affinity purification-mass spectrometry (AP-MS), tandem affinity purification (TAP) tagging, fluorescence resonance energy transfer (FRET) and co-immunoprecipitation.
Course Synopsis
The overarching goal of this course is to provide students with a thorough overview of both the theoretical and experimental aspects of structural and functional genomics as well as proteomic
Course Learning Outcomes
Students would be able to understand basic concepts of genome organization and genetic mapping. Students will also learn principles of recent sequencing technologies and spectrometry method. This course will provide opportunity to students to learn wide-range of genomics and proteomics applications.
Genome organisation
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genetic mapping (RFLP, microsatellite, SNP); high-resolution physical mapping (STS, EST)
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Flow cytometry
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Artificial chromosomes
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Whole genome shotgun sequencing
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high-resolution physical mapping
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flow cytometry
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somatic cell and radiation hybrids
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artificial chromosomes in bacteria and yeast
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hierarchical and whole genome shotgun sequencing
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DNA sequencing strategies - manual and automated sequencing
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pyrosequencing
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Solexa Sequencing
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Helicos Sequencing
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Real-time Sequencing
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Nanopore sequencing
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Sequence assembly, obstacles and solutions
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Estimating gene number
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Homology searches
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exon prediction programs
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integrated gene-finding software packages
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Structural variation in the genome and its applications
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microarray and RNA interference
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Proteomics
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cellular communication/signaling pathways
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protein-protein interactions and validation - yeast two hybrid system
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tandem affinity purification (TAP) tagging
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fluorescence resonance energy transfer (FRET)
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co-immunoprecipitation
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Book Title : Functional Genomics and Proteomics: Basics, Opportunities and Challenges
Author : NIKOLAI KLEY, STEFAN SCHMIDT, VIVIAN BERLIN, HANNES LOFERER, JENO GYURIS
Edition :
Publisher :
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Title : Genomics, Proteomics, and the Changing Research Environment
Type : Other
View Genomics, Proteomics, and the Changing Research Environment
Title : Genome Organization and Structure
Type : Other
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Title : Positional Candidate Genes for Congenital Chloride Diarrhea Suggested by High-resolution Physical Mapping in Chromosome Region 7q31
Type : Other
View Positional Candidate Genes for Congenital Chloride Diarrhea Suggested by High-resolution Physical Mapping in Chromosome Region 7q31
Title : Current challenges and solutions of de novo assembly
Type : Other
View Current challenges and solutions of de novo assembly
Title : GeneAlign: a coding exon prediction tool based on phylogenetical comparisons
Type : Other
View GeneAlign: a coding exon prediction tool based on phylogenetical comparisons
Title : Affinity purification-mass spectrometry
Type : Other
View Affinity purification-mass spectrometry