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Course Contents

COURSE CONTENTS THEORY 1. Principle of Spectroscopy (Types: UV Spectroscopy, IR Spectroscopy, Flame Atomic Spectroscopy, Spectrofluorimetry). Nature of rays. Applications 2. Direct potentiometry and pH meter 3. Principle of Chromatography (Types: Thin layer Chromatography (TLC), Column Chromatography, Paper Chromatography, Gas Chromatography, Liquid Chromatography, Affinity Chromatography, Ion-exchange Chromatography, Size exclusion Chromatography, HPLC) 4. Principle of Centrifugation, types and applications 5. Polymerase Chain Reaction 6. Principle of Electrophoresis (Types: Paper Electrophoresis, Polyacrylamide gel Electrophoresis, Agarose gel Electrophoresis). Applications 7. Enzyme-Linked Immunosorbent Assay (ELISA), Microarray Technology, DNA Sequencers, Lyophilizer, Microtomy 8. Extraction and quantification of Nucleic Acids PRACTICALS 1. Observation and understanding of a Spectroscope, Centrifuge machine, Thermocycler, Lyophilizer, Microtome machine 2. Handling of pH meter, adjusting zero error and performing pH measurement 3. Separation of mixtures by paper chromatography 4. Bio assay using Enzyme-Linked Immunosorbent Assay (ELISA) 5. Extraction and quantification of Nucleic Acids 6. Preparation of buffers and separation of nucleic acids on agarose gel by electrophoresis

Course Learning Outcomes

Develop skill and knowledge to select and use the appropriate spectroscopic, chromatographic and electrochemical instruments for specific analysis.

Gel electrophoresis

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Microarray technology

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Microarray technology - 2nd part

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DNA sequencer -introduction to apparatus

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DNA sequencing- Sangers method

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Book Title : Fundamentals and Techniques of Biophysics and Molecular Biology
Author : Pranav Kumar, Usha Mina
Edition : 2nd
Publisher : Pathfinder Publication
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Title : Lecture 1 Gel Electrophoresis
Type : Presentation

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Title : Lecture 2
Type : Presentation

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Title : Lecture 3
Type : Presentation

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