Course Contents

TEACHING AND LEARNING STRATEGY 1. Lectures: 45 - 48 2. Discussions / Tests / Assignments: Regular 3. Field trips/ Documentaries Regular COURSE CONTENTS Principles of physical, chemical and microbiological analysis of environmental pollutants. Personal Protective Equipment (PPE) at work. Laboratory Practices, Safety Symbols, Safety Contract, Safety Equipment. Basic operations in environmental laboratories (chemical reagents purity, standard, and reference materials). sampling rules, sample collection and preservation. Laboratory Techniques and Field Monitoring for parameters of importance causing environmental pollution. What is safe level, Sampling and sampling variability: Representative sampling, Samples storage and Critical path and critical group . Transport of pollutants in the environment and approaches to their analysis: Bio-magnification and Degradation, Transport and re¬concentration of, metal ions: Solubilization, Deposition and uptake by organisms. What is safe level, Sampling and sampling variability: Water analysis-Major constituents: Sampling, Techniques for analysis of common ions; UV/visible spectrometry, Emission spectrometry (Flame photometry), lon-chromatography and Examples of uses of other techniques. Water analysis-Trace pollutants: Organic trace pollutants; Sampling and storage, Extraction techniques for chromatographic analysis, Gas chromatography, Liquid chromatography, immunoassay and Spectrometric methods. Metal ions; Storage of samples, Pre-treatment, Atomic spectrometry, Visible Spectrometry. Ultra-trace analysis: Analytical methods; Mass spectrometry detection, Quantification, Gas chromatography and Examples. Quality control and quality assurance. LAB WORK 1. Extraction of chlorophyll from plant material. 2. Analysis of water, waste water sample by visible spectrometry. 3. Quantification of lead in industrial wastewater. Using AAS 4. Analysis of waste water by HPLC 5. Separation of neutral ,acidic/basic organic components by using Separatory funnel

Course Learning Outcomes

The objectives of this course are to impart skills for sampling techniques and identification of sampling locations where high molecular-mass organic compounds and metals may accumulate, provide understanding about sampling and sample variability in air, water, land, solid and waste analysis, impart skills for the range of the methods needed for subsequent chemical analysis i.e., from classical to instrumental methods including Mass spectrometer, UV/visible spectrophotometer, Infrared spectrometer, NMR, GC-MS, HPLC etc. and train for methods to interpret the result of analysis and quality assurance.