Course Contents
• Biology to Biotechnology, Ancient and Modern biotechnology; Basic Principles of recombinant DNA Technology; New diagnostic tools; Protein engineering.
• Exploiting of Microbes; Microbial culturing, isolation and identification, Preservation and growth measurements, Contamination and its remedial measures, Fungal culturing; Isolation and preservation.
• Plant Biotechnology; Plant tissue culture techniques; Biotechnology in Plant production -secondary metabolites, Agriculture and Forestry.
• Animal Biotechnology; mammalian cell culture and their application. Transgenic animals. Clones, Medicinal Plants, Genetically manipulated food. Ribo-typing, Cloning vectors, Restriction enzymes, DNA/RNA extraction and quantification (Animal, Plant); quantitative and qualitative PCR and genotyping.
• Biotechnology in human health; Basis of disease inherited disease; pathology of vascular, brain disease; current therapy, Gene therapy, current trends in the manufactured foods.
• FPLC, HPLC, 2.D gel electrophoresis
Books Recommended:
• Molecular Biotechnology by S.B.Primrose, Black well scientific Publications. 1991.
• Biotechnology, Biosafety and Biodiversity, 1999, Shanthanam,S and Montgomery, J.F.,Oxford &I.B.H. Publishing Co.
• Basics of Biotechnology by S.Ignacimuthu (2001). Tata Mcgraw-Hill Company Limited, New Dehli, India.
• Plant Biotechnology by S.Ignacimuthu (1997). Science publishers.
pure cultures isolation techniques
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DNA extraction and quantification
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preservation of pure culture
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preservation of pure culture
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Plant Biotechnology and Molecular Markers
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Restriction enzymes
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Polymerase chain reaction
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Real-Time PCR
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RNA Extraction and quantification
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preservation of cultures
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preservation-of-bacterial-and-fungal-strains
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Ribotyping
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genotyping
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Microbial preservation and growth measurements
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MICROBIAL GROWTH
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Title : Ribotyping
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Title : Media and explant preparation for plant tissue culture
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Title : Plant Tissue Culture
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Title : Lec4- Microbial Growth Measurements
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Title : Microbial growth measurements
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Title : Fungal culturing, isolation and preservation
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Title : Contamination and its remedial measures,
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Title : PLANT BIOTECH AND MOLECULAR MARKERS
Type : Other
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Title : MOLECULAR MARKERS AND PLANTS
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Title : PLANT MOLECULAR
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Title : PLANT VIROLOGY 1
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Title : PLANT VIROLOGY 2
Type : Other
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Title : MICROBIOLOGY
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Title : MS MICROBIOLOGY
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