Course Contents

CONTENTS: • Introduction: An introduction to enzymes, Brief history, Nomenclature, Classification of enzymes. • Structure of enzymes, Monomeric and oligomeric enzymes. • Specificity of enzyme action, • Enzyme Kinetics: Kinetics of single substrate enzyme catalysed reactions- Michaelis-Menten equation, Significance of Km, The Line weaver-Burk plot, Turn over number, Kinetics of multi substrate enzyme catalysed reactions- Ping-Pong bi-bi mechanism, Random order mechanism, Compulsory-order mechanism, Pre Steady state kinetics, Steady state kinetics. • Enzyme Inhibition: Competitive inhibition, Uncompetitive inhibition, Non-competitive inhibition, mixed inhibition, Partial inhibition, Substrate inhibition, Allosteric inhibition and Irreversible inhibition. • Enzyme Extraction and Purification: Extraction of soluble enzymes, membrane bound enzymes, Preliminary purification procedures, Advanced purification procedures. • Clinicaly Relavent Enzymes: Application of enzymes in medicine, Lactate dehydrogenase, Alkaline phosphatase, Acid phosphatase, Ornithine carbamoyltransferase. • Enzyme Immobilization: Physical and chemical techniques for enzyme immobilization-Adsorption, Matrix entrapment, Encapsulation, Cross-linking, Covalent bonding, Advantages and disadvantages, Application of immobilized enzymes. Enzyme Engineering: Introduction PRACTICALS: • Extraction of extracellular enzymes. • Preparation of standard curve of xylose • Estimation of the activity of amylase. Estimation of the activity of xylanase. • Evaluation of optimum temperature for enzyme activity. • Evaluation of optimum pH for enzyme activity. • Study of time course for amylase activity. Books Recommended: ¬ David L. Nelson and Michael M. Cox  2012 Lehninger Principles of Biochemistry 6th edition. ¬ Syed Tanveer Ahmed Inamadar 2013 Biochemical engineering principles concepts 3rd edition PHI Learning Private Limited; ¬ Athel Cornish .2012.Fundamentals of Enzyme Kinetics 4th edition Willy-VCH verlag GmbH pres ¬ Palmer T., Philip L. R. And Bonner. 2007. Enzymes: Biochemistry, Biotechnology, Clinical Chemistry, Horwood. ¬ Basic Biotechnology, 2006 Second Edition, By Colin Ratledge and Bjorm Kristiansen, Cambridge University Press. ¬ Daniel L purich Enzyme Kinetics: Catalysis and Control: A Reference of Theory and Best-practice Methods Ist edition 2010 Elsevier Science Publishing Co Inc

Course Synopsis

To develop skills of the students in the area of Enzyme Engineering and Technology The students would have learnt about classification of enzymes, immobilization, extraction and purification and application