Course Contents

• Laminar air flow and Biosafety cabinets • pH meter • Centrifuges (Micro as well as macro) • Gel Electrophoresis • Gel Doc system • Hybridizer • Biolistic gun/ gene gun • Spectrophotometer (UV, visible and IR) • DNA Sequencer • Freeze dryer • HPLC • FPLC • MALDI-TOF • Spectroscopy • Separation techniques • Isotopes • Restriction maps • Blotting and hybridization • Sequencing • Protein-DNA interation. Books Recommended: • Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman. J.G., Smith, J.A.and Struhl, K., 2003. Current Protocols in molecular biology.John Wiley and Sons, New York. Sambrook, J., Fritsch, E.F., and Maniais, T., 1889. Molecular Cloning. A laboratorymanual. Cold Spring Laboratory Press, Cold Spring Harboor, New York

Course Synopsis

This course deals with working principal, calibration, demonstration, uses and precautions for using each of the following instruments.