Course Contents
Definition and introduction of DNA Recombinant Technology.
• Importance and Applications of DNA Recombinant Technology in industrial, agricultural, biomedical and environmental fields with comprehensive theoretical and practical knowledge.
• Different approaches to isolate Nucleic acids, Cleavage of DNA, Genetic maps, Specificity of restriction fragments; cloning vectors, various types; making new DNA molecules,
• Gene libraries and CDNA cloning.
• Different means of introducing DNA into Prokaryotes and Eukaryotes, Identification and screening of recombinant DNA molecules.
• Characterization of genes, verification of gene identity, use of transposable elements; determination of gene structure,
• Gene expression and understanding of its regulation.
• DNA-protein interactions, fusion of regularity regions to reporter genes. Construction of chromosomal integrates in transgenic plants and animals.
Practicals:
Practicals related to theory would be demonstrated and conducted.
Course Synopsis
• To offer the students a good understanding of the basic principles of recombinant DNA Technology.
• To make students understand the importance of this advanced research based course in practical fields of today’s life.
• To recognize the role of recombinant DNA technology in contributing to both basic and applied research in industry, medicine, agriculture, pharmacy and food technology.
• To prepare the young generation to enter in most emerging industry of hybrid technologies and GMOs
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Book Title : Recombinant DNA and Biotechnology
Author : Helen Kreuzer and Asrianne Massy
Edition : 2000
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