Course Contents
Culturing and isolation of bacteria
Streaking of bacteria on solid medium, serial dilution preparation
Colony count and cell count of bacteria
Long term Storage of bacterial cultures
Extraction of Genomic DNA
Extraction of Plasmid DNA
Quantification of DNA by spectrophotometer
Running DNA on Agarose gel
Comparing plasmids of different molecular weights using molecular weight markers.
Course Synopsis
This course includes Advance molecular biology practicals.
Course Learning Outcomes
Students will be able to culture bacteria and isolate DNA and use it further in their experiments (Running DNA on Agarose, Spectrophotometer quantification, PCR)
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Book Title : Cell and Molecular Biology concepts and Experiments
Author : Gerald Carp
Edition : 5th edition
Publisher : John Willey and Sons Publications.
Book Title : Molecular Biology Lab
Author : Brown T. A.
Edition : 1998
Publisher : Academic Press Publication
Book Title : Methods in Biotechnology and Bio-engineering.
Author : Vyas SP and Kohli
Edition : 5th edition
Publisher : CSB Publishers.
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