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Course Contents

Basic processes of molecular biology: transcription; translation, post translational modifications, reverse transcription (retrovirises) enzymes involved in the processes. Gene expression and its regulation in prokaryotes and eukaryotes, proofreading and repair mechanisms. Mitochondrial genome. Cutting and ligation of DNA, Genetic manipulation vectors, PCR its types and applications.

Course Synopsis

This course contains a summary of various molecular biology processes,Cutting and ligation of DNA, Genetic manipulation vectors, PCR its types and applications.

Course Learning Outcomes

Students will learn about the post translational modifications/ post transcriptional modifications.Mitochondrial genome. Cutting and ligation of DNA, Genetic manipulation vectors, PCR its types and applications.


Restriction endonucleases

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Ligation of DNA , Linkers and double linkers

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Difference between linkers and adaptors

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Klenow fragment

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DNA sequencing

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RAPD/RFLP, AFLP

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Sanger sequencing method - dideoxy sequencing of DNA

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Maxam gilbert DNA sequencing method

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Book Title : Gene X
Author : Brown T. A
Edition : 2016
Publisher : Academic Press Publication







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