Course Contents

Introduction to the aquaculture biotechnology: Applications of biotechnology in aquaculture, Scope for use, Public perception, interdisciplinary pursuit biotechnology and developing world. Role of biotechnology in marine and aquaculture pollution control. Bio-Remediation, induced breeding of fish, Cryopreservation Recombinant DNA and genome analyses: gene manipulation, molecular cloning. Application of DNA technology in aquaculture. The determination of base sequence in DNA, allopheny, Methods of transfection, Electroporation, Protoplast fusion. Liposomes as vectors for gene transfer, Requirement for gene expression. Expression of aquaculture genes in prokaryotic and eukaryotic systems. Genetic engineering and DNA cloning: gene targeting approaches in biotechnology, isolation of nuclear and extra nuclear DNA, gene modification; introduction to Southern, northern and south-western blots; hybridization, colony hybridization and sequencing of proteins and nucleic acids; Polymerase Chain Reaction (PCR). Optimization of PCR conditions General principles and strategies for cloning, Cloning tools, The enzymology of in vitro DNA recombination, Vectors used for cloning, Sythesis of DNA for cloning, Reaching the gene via mRNA and protein, Synthesis of complete gene, Reporter genes, Recombinant virus genes. Transgenic Fish. Expression of cloned genes in aquaculture. Triploidy, Chromosome Engineering Cell and tissue culture: Basic techniques of cell culture and their applications, primary and secondary cultures, cell lines, cell clones, somatic embryogenesis, haploidy, protoplast fusion, somatic hybridization and cybrids. Molecular biological techniques for rapid diagonosis of diseases in Aquaculture: Mapping genetic diseases in fish. Spectrophotometeric and UV spectroscopic analysis of nucleic acids, Agarose gel electrophoresis, Pulsed/field gel electrophoresis, Hybridization, Foot printing, Reverse transcriptase, Site directed mutagenesis, Restriction fragment length polymorphism (RFLP), 2 - dimensional gel electrophoresis. DNA finger printing, Enzyme linked immunosorbant assay (ELISA), Diagnostic Polymerase PCR, Rapid amplification of cDNA ends (RACE), Other applications of PCR, Gene therapy. Vaccine for aquaculture species Biosafety Regulation: Introduction to genetically manipulated organisms (GMOs), Release of genetically engineered organisms in the environment, Biosafety management, Debate on the safety work in biotechnology. BOOKS RECOMMENDED: 1. Principles of Gene Manipulation and Genetics. Primrose, S.B. and Twyman, R. M. 2006. 7th edition. Blackwell Publishing, USA. 2. Introduction to Genetic Analysis. Griffiths, A. J. F., Wessler, S.R.., Lewontin, R.C. and Carroll, S. B. 2008. 9th edition. W.H. Freeman and company, USA, New York. Basic Biotechnology. Ed. Ratledge, C. and Kristiansen, Bjorn. 2006. 3rd edition. Cambridge University Press, New York. 3. Genetics: From Genes to Genomes. Hartwell, L.H., Hood, L., Goldberg, M.L., Reynolds, A.E., Silver, L.M. and Veres, R.C. 2008. 3rd edition. McGraw-Hill Companies, Inc. Avenue of Americans, New York. 4. “Marine Micribiology” Litchfield CD DHR 5. “Application of genetics in aquaculture” Bye & Ponnaiah CMFRI 6. “Biotechnology” Travan et al Tata-NcGraw

Course Learning Outcomes

The use of modern biotechnology to enhance production of aquatic species holds great potential not only to meet demand but also to improve aquaculture. To support the rapid growth of aquaculture and to strike a balance between best possible production and minimum harm to the environment, it has become crucial to integrate biotechnology into aquaculture. This course will acquaint students with the fundamental principles of aquaculture biotechnology.