Course Contents

TEACHING AND LEARNING STRATEGIES 1. Lectures 30-32 2. Assignments/ Presentations one per student AIMS AND OBJECTIVES • Describe the basic techniques used in recombinant DNA technology. • Explore the practical uses of genetic engineering. • Consider potential problems related to genetic engineering. COURSE CONTENTS • Introduction and scope. • Restriction and modification system. • Properties of restriction endonucleases, their occurrence and recognition sequences. Assay procedures for restriction endonucleases and slab gel electrophoresis. Practical uses of endonucleases. Role in genetic engineering. • Exchange of genes between a lambda phage and Col EI factor. • In vitro genetic engineering; cloning vehicles: plasmids, cosmids and phagemids, YAC and BAC etc. • Principles of nucleic acid isolation (DNA & RNA). • Cloning strategies: construction of chimera plasmids. • Methods of introducing exogenous DNA. • Methods for screening the clones. • DNA sequencing. • PCR: its application and primer designing. • Expression systems for Pro- and Eukaryotes. • Markers for labeling methods of probes. • Concept of construction of genomic library. Books Recommended 1. Robert F.W., 2018. Molecular Biology . McGraw-Hill. 1. Dale, J.W. and von Schantz , M. 2002. From Genes to Genomes: Concepts and Applications of DNA Technology . John-Wiley and Son Limited. 2. Meyers, R.A., 2010. Genomics and Genetics. John-Wiley and Son Limited. 2. Primrose, S.B., and Twyman, R.M. 2006. Gene Manipulation and Genomics 6th edition. Blackwell Publishing