Course Contents

Preparation of Murashige and Skoog medium and stocks of macronutrients, micronutrients, and hormones; selection of ex-plant, medium preparation and callus induction; culturing Agrobacterium and using it to infect plant callus; selection of transformants; regeneration of plantlets and acclimatization; plant DNA extraction and PCR for detecting introduction of foreign DNA into plants.

Course Synopsis

To acquaint students with techniques and skills employed for producing transgenic crops.