Course Contents

Basic biochemical methods such as iodine test for polysaccharides, fermentation of sugars by Baker’s yeast; isolation of amylose and amylopectin from starch; extraction of glycogen from liver; acid and enzymatic hydrolysis of glycogen; extraction and estimation of lipids from plant tissue/seed and lipid separation from different tissues; fractionation by thin layer chromatography (TLC). estimation of blood cholesterol; estimation of lipid soluble vitamins; fractionation of brain lipids; titration and separation of amino acids; peptide sequencing; protein quantification;

Course Synopsis

This course is designed to make the student able to turn over the abstract knowledge to practical purposes. This course emphasizes the isolation, estimation and characterization of different biological samples and study of some properties of amino acids, proteins, sugars, lipids and nucleic acids. The course shall include extraction, fractionation and characterization of these biomlecules.