Course Contents

Definition and introduction of DNA Recombinant Technology. • Importance and Applications of DNA Recombinant Technology in industrial, agricultural, biomedical and environmental fields with comprehensive theoretical and practical knowledge. • Different approaches to isolate Nucleic acids, Cleavage of DNA, Genetic maps, Specificity of restriction fragments; cloning vectors, various types; making new DNA molecules, • Gene libraries and CDNA cloning. • Different means of introducing DNA into Prokaryotes and Eukaryotes, Identification and screening of recombinant DNA molecules. • Characterization of genes, verification of gene identity, use of transposable elements; determination of gene structure, • Gene expression and understanding of its regulation. • DNA-protein interactions, fusion of regularity regions to reporter genes. Construction of chromosomal integrates in transgenic plants and animals.

Course Synopsis

• To offer the students a good understanding of the basic principles of recombinant DNA Technology. • To make students understand the importance of this advanced research based course in practical fields of today’s life. • To recognize the role of recombinant DNA technology in contributing to both basic and applied research in industry, medicine, agriculture, pharmacy and food technology. • To prepare the young generation to enter in most emerging industry of hybrid technologies and GMOs.