Course Contents
Basic Molecular Genetics Mechanisms:
Structure of nucleic acids, DNA replication, RNA & Transcription, Protein synthesis.
Mutagenesis and Cancer:
Introduction to mutagenesis, Site directed mutagenesis, Tumor including Ti plasmids, Oncogenes and Cancer.
Gene Cloning and Sequencing:
An outline of DNA cloning methods, Cloning vectors including plasmids, bacteriophages, Cosmids and expression vectors, Gene splicing, Chromosome walking, Development of gene libraries, Gene sequencing, Potentials of recombinant DNA technology.
DNA Amplification and Gel Electrophoresis:
Introduction to DNA amplification and polymerase chain reaction (PCR), Gel electrophoresis of DNA, Pulse-field agarose gel electrophoresis.
Transgenic Proteins, Organisms and Gene Therapies:
Production of transgenic proteins, transgenic organisms and gene therapies, Restriction fragment length polymorphisms (RFLP) and disease detection.
Molecular Genomics:
Molecular definition of a gene, Analysis of gene structure and expression, inactivating the function of specific genes in eukaryotes, Human genome project.
Practicals:
Practicals related to course will be demonstrated and conducted
Course Synopsis
• To offer the students a good understanding of the basic principles of Molecular Biology applicable to all living organisms.
• To make students realize that it is a subject of practical importance, contributing to both basic and applied research in , medicine, agriculture, pharmacy and food industry etc.
• To recognize the role of Molecular Biology in contributing to both basic and applied research in all aspects of life.
• To consolidate student’s training in Molecular Biology and related disciplines and encourage them to develop intellectual independence, critical thinking skills and versatility for problem solving.
• To develop the practical abilities of students to enhance their observational skills and to use these skills for problem solving in practical life .
No Information Yet
Book Title : Genetics: Analysis Of Genes And Genomes
Author : Hartl D .L
Edition : 2019
Publisher :
Book Title : Basic and Molecular Genetics.
Author : Tomar A. K
Edition : 2018
Publisher :
Book Title : Molecular Biology: Structure And Dynamics Of Genomes And Proteomes
Author : Zalatanova
Edition : 2016
Publisher :
Book Title : Gene Cloning And DNA,Analysis: An Introduction
Author : Brown T
Edition : 2016
Publisher :
Book Title : Basic Techniques In Biochemistry And Molecular Biology
Author : Das.L
Edition : 2014
Publisher :
Book Title : Genetics.
Author : Benjamin A. Pierce
Edition : 2015
Publisher : W.H. Freeman and Company
Book Title : Molecular Cell Biology
Author : Harvey Lodish .
Edition : 2014
Publisher :
Book Title : Essentials of Molecular Biology
Author : David Freifelder
Edition : 2000
Publisher :
No Information Yet