Course Contents
General introduction
1. Plant metabolites
1- Primary metabolites
2-Secondary metabolites
How and why these compounds are synthesized by plants, good and bad uses of these compounds by humans, Biosepration of compounds
3. Primary Bioassay screening
3.1 Toxicity Assays
3.1.1 Brine –shrimp lethality assay
3.1.2 Brine –Shrimp micro well cytotoxicity assay
3.1.3 Potato tumor inhibition assay
3.2 Antimicrobial Assay
3.2.1 Antibacterial assay
3.2.2 Agar diffusion assay
3.2.3 Agar dilution assay
3.2.4 Direct bioautobiography method
3.3 Antifungal Assay
3.3.1 Agar tube dilution assay
3.3.2 Direct bioautobiography method.
3.4 Antiviral and Anticancer Assay
3.4.1 Anti-HIV assay
3.4.2 Anticancer assay
1.4. Hepatotoxicity assays.
(i) Antihepatotoxic activity using carbon tetra chloride induced cytotoxicity
(ii) invivohepatoprotectiveassay
1.5. Hypoglycemic/Antidioabetic activity assay
1.6. Diuretic activity assays
1.7. Anthelmintic activity assays
1.8. Anti inflammatory assay
1.9. Antiepileptic
1.10. Analgesic assay
Practicals
• Isolation of plant extracts
• Designing of different bioassays techniques mentioned in Syllabus
Bioassay techniques
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Primary metabolites
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Secondary metabolites
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Bioseperation
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Brine shrimp lethality assay
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Cytotoxicity assay
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Antimicrobial assay 1
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Antimicrobial assay 2
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Disc diffusion assay (A)
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Disc diffusion assay (B)
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Agar dilution assay (A)
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Agar dilution assay (B)
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Agar dilution assay (C)
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MIC
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Antifungal assay 1
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Antifungal assay 2
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Action of Antifungal drug
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Anticancer assay 1
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Anticancer assay 2
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Anticancer assay
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Antiviral assay
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Antiviral assay 1
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Hepatotoxicity assay
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Hepatoprotective assay
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Elisa
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Elisa 1
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Antidiabetic activity assay
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Hypoglycemic activity assay
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Anti-diabetic assay
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Diuretic activity assay
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Diuretic activity assay
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Anthelmintic activity assay
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Anthelmintic activity assay 1
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Anthelmintic activity assay 2
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Anti inflammatory assay
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Anti inflammatory assay 1
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Antiepileptic assay
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Antiepileptic assay 1
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Analgesic assay
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Analgesic assay 1
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Book Title : Bioassay techniques for drug Development
Author : Atta-urRahman and M.Iqbal Chaudhary.
Edition : 2001
Publisher : Taylor and Francis London.
Book Title : Natural products from plant
Author : Peter B. Kaufman Leland J. Cseke Sara Warber, James A. Duke
Edition : 1999
Publisher : CRC. Press LLCUsa
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