Course Contents

TEACHING AND LEARNING STRATEGIES 1. Lectures 30-32 2. Assignments/ Presentations one per student AIMS AND OBJECTIVES The continuity of the life from one generation to other generation is based on the mechanisms involving nucleus, chromosomes and genes etc. COURSE CONTENTS Nucleic acids structure and functions. DNA replication: replicon origins, events that occur at the replication fork, the structure and functions of DNA polymerases, and replication strategies. Control of DNA replication: dichotomous replication in prokaryotes. Control of gene expression in prokaryote: polycistrons, transcriptional initiation and termination, the operon, catabolite repression and attenuation control. Protein synthesis - mRNA translation: Genetic code - non universality, codon usage. Events on ribosomes (c.f. prokaryotes), ribosome structure-function relationships, organelle and archaebacterial systems. Plasmids, episomes and transposons. DNA mutagenesis and mutagenic agents, repair and mutation suppression. Genetic recombination: generalized recombination, site specific recombination and Illegitimate recombination. Gene transfer mechanisms and their role in evolution. Transformation, transduction, conjugation and cross-phylogenic transfer. Gene mapping by conjugation and transduction. Circular chromosomal maps of bacteria. Introduction to genetic rearrangements. Books Recommended 1. Pierca, B. A., 2005. Genetics. A conceptual approach, W. H. Freeman and Company, NY. 2. Griffiths, A. J. F., Wessler, S. R., Lewontin, R. C., Gelbart, W. M., Suzuki, D.T. and Miller, J. H., 2005, Introduction to Genetic Analysis, W. H. Freeman and Company. 3. Hartl, D. L. and Jones, E. W., 2005, Genetics - Analysis of Genes and Genomes, Jones and Bartlett Publishers. Sudbury, U.S.A. 4. Ignacimuthu, S., 2005, Basic Bioinformatics, Narosa Publishing House, India. 5. Lwein, B., 2004, Gene VIII, Pearson Education Int. 6. Primrose, S. B., and Twyman, R. M., 2006. Principles of Gene Manipulation and Genomics. Blackwell Scientific Publications. 7. Wilson, J. and Hunt, T., 2004. Molecular Biology of the cell – the problems book, Garland publishing Inc. 8. Jack, J. P., 2005. An Introduction to Human Molecular Genetics, 2nd edition New Jersey. 9. Ringo, J., 2004. Fundamental Genetics, Cambridge University Press.