Course Contents

TEACHING AND LEARNING STRATEGIES 1. Lectures 30-32 2. Assignments/ Presentations one per student AIMS AND OBJECTIVES • Describe the basic techniques used in recombinant DNA technology. • Explore the practical uses of recombinant DNA technology. • Consider potential problems related to Molecular techniques and recombinant DNA technology. Contents 1. SALIENT FEATURES OF CLONING VECTORS a. Types of cloning vectors b. Plasmids, cosmids, Yeast cloning vectors c. Animal viruses, Ti plasmids . 2. PLASMIDS a Structural and functional organization of plasmids b. Plasmid replication c. Stringent and relaxed plasmids d. Incompatibility of plasmid maintenance - 3. GENE MODIFICATION & APPLICATION OF RECOMBINANT DNA TECHNOLOGY a. Mutagenesis-Deletion mutagenesis, Oligonucleotide derived mutagenesis, Site directed mutagenesis b. Applications of rDNA technology in Diagnostics c. Molecular probes (Production, labelling and uses) - 4. ENZYMES IN GENETIC ENGINEERING a. DNA polymerase, polynucleotide kinase, T4 DNA ligase b. Nick translation system c. Terminal deoxynucleotidy1 transferase, Reverse transcriptase, Restriction endonucleases Type I & II. Genomic and cDNA libraries, screening methods for gene libraries Southern and Northern blotting; Potentials of recombinant DNA technology; PCR; production of proteins,transgenic organisms and gene therapies Books Recommended 1. Basic Biotechnology. C. R. Attedge, and B. Kristiansen, 2001. Cambridge University Press, U.K. 2. A Manual of Basic Technique. Freshney, R. Ian. 2000. Alan R. Liss, Inc., New York. 3. Application of Microbes in Biotechnology. Lee, Y.K. & C.L. Poh and H. M. Tan.1999. Springer-verlag