Course Contents

1. Introduction to cell biology. 2. Difference between prokaryotes and eukaryotes. 3. Physico-chemical properties of protoplasm. 4. Ultra-structure, chemical composition and functions of cell wall, cell membrane, cellular organelles (mitochondria, endoplasmic reticulum, golgi apparatus, lysosome, glyoxysome, nucleus, ribosomes, etc.) cytoskeleton. 5. Chemical composition and molecular structure of chromosomes. 6. Cell cycle and apoptosis. 7. Cell reproduction. 8. Signal transduction. 9. Cell culture 10. E. coli and yeast as representative prokaryotic and eukaryotic models for molecular differentiation. 11. Chromosome Structure and Function 12. Molecular mechanism of Replication. 13. Transcription and Translation. 14. Transcriptional and translational regulation of gene expression. 15. Regulation of gene expression in prokaryotes and eukaryotes. 16. Types of recombination. 17. Mutations and chromosomal aberrations. 18. DNA damage and repair. 19. Gene sequencing. 20. Principles of Recombinant DNA technology. 21. Role of Recombinant DNA Technology in economic development. 22. Human Genome Project. 23. Stem Cell Research. Practicals: 1. Karyotyping. 2. Study of DNA damage by physical and chemical methods. 3. Case study of chromosomal abnormalities in human and agricultural specimen. 4. Ames test for identification of mutagenic agents. Recommended Books 1. De Robertis, E. D. P and De Robertis Jr. E. M. F. 2001. Cell and Molecular Biology.8th Edition. Lippincott, Williams and Wilkins Publishers. 2. Karp, G. 2000. Cell and Molecular Biology. Concepts and Experiments. John Wiley and Sons Publishers. 3. Lodish, H. 2001. Molecular and Cell Biology. W. H. Freeman and Co. 4. Gilmartin, P. M. and C. Bowler. 2002. Molecular Plant Biology. Vol 1 and 2. Oxford University Press. UK. 5. Malacinski. G. M. 2003. Essentials of Molecular Biology. 4th Edition. Jones and Bartlett Publishers, Massachusetts.

Course Synopsis

Advanced cell and molecular biology course to BS students will be taught by using: 1. Powerpoint presentations. 2.Links. 3. Reference books Topics for assignments will be given and will be evaluated by presentations.

Course Learning Outcomes

The cell and its organization of architecture and the unified role it plays for the ultimate sustainability of the organisms. The various ultra-structural, molecular and functional aspects of the cells.