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Maj/Bot-416  : PLANT BIOTECHNOLOGY

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Course Contents

A. History and Introduction: • Introduction related to its different practical applications. B. Crop Production and Protection: • Transformation. Role of Somatic hybridization in Transformation, Methods and Techniques. • Vector Construction. Different types of Tranformation vectors. Methods of vector construction in relation with in vitro cultures. • Transformation.Role of plant cell culture in DNA Tranformation. • Role of Transgenic plants in crop protection. • Biopesticides for use in agriculture and health sector. C. Gene Transfer System for Plants: • Agrobacterium mediated Transformation. Different steps of Agrobacterium mediated transformation. • Plant Viral Vectors. Types and methods of construction of viral vectors.Applications. • Direct Transformation. Protoplasmic and non protoplasmic direct Transformation. D. Bioconservation: • Microbial Biotechnology for detoxification of industrial effluents and pesticide waste. • Lignocellulose Biotechnology and Recycling of Cellulose Biotechnology. • Microbial protein production. • Fermentation. Practicals Experiments related to Plant cell culture, Protoplast isolation with reference to biotechnological aspects, Transformation (chemical and agrobacterium mediated). Experiments showing Bioconservation with respect to Enzymology and different Fermentation techniques.

Course Synopsis

This course explores the use of biotechnology to both generate genetic variation in plants and to understand how factors at the cellular level contribute to the expression of genotypes and hence to phenotypic variation. There is an emphasis on the molecular mechanisms directing plant gene expression under diverse environmental and developmental stimuli. This knowledge is central to our ability to modify plant responses and properties for global food security and commercial gains in biotechnology and agriculture. A problem-based learning approach is employed to demonstrate the use of various omics technologies (such as genomics, proteomics and metabolomics) to gain this knowledge. In the laboratory classes, students will perform some of the techniques currently used to generate information and detect genetic variation. The key concepts of the course will be integrated in a series of case studies, and students will enhance their ability to apply them to novel situations in problem-solving sessions and their own research planning project.

Course Learning Outcomes

Successful students will be able to: 1. explain the basics of the physiological and molecular processes that occur during plant growth and development and during environmental adaptations 2. understand how biotechnology has been used to develop knowledge of complex processes that occur in the plant 3. use basic biotechnological techniques to explore molecular biology of plants 4. understand the processes involved in the planning, conduct and execution of plant biotechnology experiments 5. explain how biotechnology is used for plant improvement and discuss the ethical implications of that use 6. communicate effectively using oral and written means for both scientific and non-technical audiences 7. cooperate and work effectively as a member of a team to solve problems 8. critically evaluate scientific research papers and develop research proposals to address identified gaps.


Plant Viral Vectors.

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Bioconservation

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https://www.researchgate.net/publication/268279657_Microbial_Biotechnology_Progress_and_Trends

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https://www.researchgate.net/publication/273433182_Advances_in_lignocellulosic_biotechnology_A_brief_review_on_lignocellulosic_biomass_and_cellulases

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Bioremediation

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lignocellulose biotechnology

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Microbial Biotechnology for detoxification of industrial effluents and pesticide waste.

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Biopesticides for use in agriculture and health sector.

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Microbial protein production

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Lignocellulose Biotechnology and Recycling of Cellulose Biotechnology

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Microbial protein production. Fermentation.

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Microbial protein production

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Bio Conservation

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Book Title : Bioremediation of Industrial Waste for Environmental Safet
Author : Gaurav SaxenaRam Naresh Bharagava
Edition : ist
Publisher : springer
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Book Title : PLANT BIOTECHNOLOGY
Author : Arie Altman Paul Hasegawa
Edition : istSPRINGER
Publisher :
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Book Title : Bioremediation of Industrial Waste for Environmental Safety
Author : Gaurav SaxenaRam Naresh Bharagava
Edition : Volume I: Industrial Waste and Its Management Editors (view affiliations)
Publisher :
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Book Title : PLANT BIOTECHNOLOGY
Author : S. Umesha
Edition : ist
Publisher :


Book Title : The Art of Fermentation: An In-Depth Exploration of Essential Concepts
Author : Sandor Ellix Katz
Edition : IST
Publisher : ONLINE
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Book Title : Plant Biotechnology and Transgenic Plants
Author : Kirsi-Marja Oksman-Caldentey, Wolfgang H. Barz
Edition : ist
Publisher :


Book Title : Plant Biotechnology and Transgenic Plants
Author : Kirsi-Marja Oksman-Caldentey, Wolfgang H. Barz
Edition : IST
Publisher : CRC PRESS
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Title : Lignocellulose biotech
Type : Presentation

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Title : Biopesticides
Type : Presentation

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Title : fermentation
Type : Presentation

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Title : lignocellulose biotechnology
Type : Presentation

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Title : Microbial biotechnology
Type : Presentation

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Title : Microbial protein production
Type : Presentation

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Title : Microbial Biotechnology
Type : Presentation

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Title : Lignocellulose biotechnology
Type : Presentation

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Title : PLANT BIOTECHNOLOGY
Type : Presentation

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Title : HISTORY OF PLANT BIOTECHNOLOGY
Type : Presentation

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Title : PLANT BIOTECHNOLOGY AND ITS APPLICATION
Type : Presentation

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Title : CROP PRODUCTION AND PROTECTION
Type : Presentation

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Title : SOMATIC HYBRIDIZATION
Type : Presentation

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Title : Transformation
Type : Presentation

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Title : Methods of Gene Transfer
Type : Presentation

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Title : VECTOR AND ITS TYPE
Type : Presentation

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Title : TRANSGENIC PLANTS AND ITS APPLICATION
Type : Presentation

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Title : AGROBACTERIUM MEDIATED TRANSFORMATION
Type : Presentation

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Title : VIRAL VECTORS
Type : Presentation

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Title : MICROBIAL BIOTECH FOR detoxification of industrial effluents
Type : Presentation

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Title : Lignocellulose biotechnology
Type : Presentation

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Title : Lignocellulose biotechnology
Type : Presentation

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Title : In vitro development pathways
Type : Presentation

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Title : microbial protien production
Type : Presentation

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