Organization and structure of genomes; genetic mapping (RFLP, microsatellite, SNP); high-resolution physical mapping (STS, EST); flow cytometry; somatic cell and radiation hybrids; artificial chromosomes in bacteria and yeast; hierarchical and whole genome shotgun sequencing; DNA sequencing strategies - manual and automated sequencing, pyrosequencing, Solexa, Helicos, Roche 454, real-time and nanopore sequencing; sequence assembly, obstacles and solutions; estimating gene number – over-prediction and under-prediction, homology searches, exon prediction programs, integrated gene-finding software packages; structural variation in the genome and its applications; microarray and RNA interference; proteomics; cellular communication/signaling pathways; protein-protein interactions and validation - yeast two hybrid system, affinity purification-mass spectrometry (AP-MS), tandem affinity purification (TAP) tagging, fluorescence resonance energy transfer (FRET) and co-immunoprecipitation.
The overarching goal of this course is to provide students with a thorough overview of both the theoretical and experimental aspects of structural and functional genomics as well as proteomics.
Course Learning Outcomes
To learn basic techniques in genomics and proteomics
Nanopore DNA sequencing
Whole genome sequencing
Book Title : Human Molecular Genetics.
Author : T Strachan; Andrew P Read
Edition : Fourth
Publisher : Garland Science