Course Contents

Organization and structure of genomes; genetic mapping (RFLP, microsatellite, SNP); high-resolution physical mapping (STS, EST); flow cytometry; somatic cell and radiation hybrids; artificial chromosomes in bacteria and yeast; hierarchical and whole genome shotgun sequencing; DNA sequencing strategies - manual and automated sequencing, pyrosequencing, Solexa, Helicos, Roche 454, real-time and nanopore sequencing; sequence assembly, obstacles and solutions; estimating gene number – over-prediction and under-prediction, homology searches, exon prediction programs, integrated gene-finding software packages; structural variation in the genome and its applications; microarray and RNA interference; proteomics; cellular communication/signaling pathways; protein-protein interactions and validation - yeast two hybrid system, affinity purification-mass spectrometry (AP-MS), tandem affinity purification (TAP) tagging, fluorescence resonance energy transfer (FRET) and co-immunoprecipitation.

Course Synopsis

The overarching goal of this course is to provide students with a thorough overview of both the theoretical and experimental aspects of structural and functional genomics as well as proteomics.

Course Learning Outcomes

To learn basic techniques in genomics and proteomics